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Keywords: Biochemistry, Issue 80, Genetics, Molecular Biology, Proteins, Proteomics, recombinant protein, affinity purification, Glutathione Sepharose Tag, Talon metal affinity resin Introduction The purification of recombinant proteins is crucial to address fundamental questions in biochemistry

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This group also demonstrated that knockdown of thioredoxin led to massive generation of general and lipid ROS in HCT 116 colon cancer cells